Screening of phytochemicals and antimicrobial activity of ethanol extract Komba-komba leaf (Chromolaena odorata) against bacteria pathogens

Yunus, Reni and Fauzi, Ahmad Zil and Fusvita, Angriani (2020) Screening of phytochemicals and antimicrobial activity of ethanol extract Komba-komba leaf (Chromolaena odorata) against bacteria pathogens. Health Polytechnic of the Ministry of Health, Surabaya. ISBN 978-623-94491-5-5

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Abstract

One of Southeast Sulawesi's local plants that has been empirically used by the community for the treat is the Komba-komba (plantChromolaena odorata). Traditionally this komb-komb plant is used as medicine f healing, skin infections. Komba-komba or kirinyuh (leavesChromolaena odorata) contain compounds that ha inhibiting the growth of microorganisms / antibacterial. The purpose of this study was to determine the a contained in the komba-komba plant (Chromolaena odorata) and to determine the antimicrobial activity of the e the kombakomba plant (Chromolaena odorata) against pathogenic bacteria. Research Methods: This typ experimental laboratories, using the One-shot Case Study design which is a research design with the treat independent. The research procedure starts from plant preparation, extract preparation, thin layer chromato inhibitory test on test bacteria. Research: ResultsThe results of phytochemical test screening of komba-komba s komba leaf extract (Chromolaena odorata) contains Alkaloids, Flavonoids, Polyphenones and tannins, saponi The inhibitory test results showed that the concentration of 1.56 ppm; 3,125 ppm; 6,250 ppm; 12,500 ppm; 25,0 ppm; and 100,000 ppm showed a zone of inhibition against the bacteria Neisseria gonnorhae, Klebsiella p Pseudomonas aeroginosa. Conclusion: Chromolaena odorata plants contain antibacterial compounds that ha inhibit pathogenic bacteria.

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